Supplemental Movie EV4. Automated tracking of immune cell migration enables high-throughput analysis of nuclear dynamics in vivo. Hemocyte nuclear (Srp>nRFP) tracks after WEKA segmentation and bespoke nuclear tracking with the MIA plugin in ImageJ/FIJI. The colour of hemocyte outline indicates the relative Shape Factor value and the track colour indicates relative speed. Overview on left with WEKA segmented nuclear probability maps and a closeup with nRFP signal on the right, all in 75h APF vessels. The images were acquired every 33 seconds; scalebars are 50 (left) and 5 (right) m.
